Jing Chang-qing
2012
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Quality indicators
Journal
Chinese Journal of Current Advances in General Surgery
Abstract
Objective: To investigate the dynamic effect of thymidylate synthase in human colorectal carcinoma LoVo cells after treatment with nolatrexed.Methods: Use the MTT method to detect the inhibition ratio after treatment with different nolatrexed concentrations in LoVo cells,RT-PCR and Western blot technique to observe the TS gene and protein expression levels,flow cytometry to detect the apoptosis rate of LoVo cells.Results: Nolatrexed can inhibit the LoVo cells proliferation significantly,the IC50 is about 6.31μmol/L.It can also promote the cells apoptosis,the apoptosis rate increased with the action time extend.The result of RT-PCR demonstrated that in the mRNA levels,TS/GAPDH ratio continue to rise all along within the time period in LoVo cells,only the ratio of 48h and 60h have significant difference to the untreatment group.But,when LoVo cells were treated with the same dose in the different time,the level of TS protein expression also keep rising as time goes on.Conclusion: Nolatrexed can significantly promote cell apoptosis and produce TS-induced phenomenon,which can better guide clinical durg use.