J. Hunter
Mar 1, 1963
Citations
0
Influential Citations
22
Citations
Quality indicators
Journal
Biochemical pharmacology
Abstract
Abstract Stimulation of aerobic acid production with 1 ppm vincaleukoblastine sulfate or 50 ppm leurocristine sulfate without inhibition of respiration was observed with thioguanine-resistant L1210 leukemia cells. To obtain a significant inhibition of respiration in time, at least 25 ppm vincaleukoblastine was required. Addition of glucose inhibited respiration of these leukemia cells (Crabtree effect) without affecting the per cent respiratory inhibition by vincaleukoblastine. Anaerobic glycolysis was not affected by 1 ppm of the alkaloid but was markedly inhibited (up to 90%) by 50 ppm. Respiratory and glycolytic inhibition by vincaleukoblastine at the concentrations studied was not affected by the absence of externally added inorganic phosphate. Monosodium glutamate (0.01 M) reduced the effect of vincaleukoblastine on aerobic acid production in line with reports of glutamic acid protection against this alkaloid with leukemia cells in tissue culture and Ehrlich ascites cells in vivo . The observations presented provide evidence that both vincaleukoblastine and leurocristine cause an inhibition of the Pasteur effect. This action of these alkaloids may affect cell energy production required for mitosis.