J. Qian, Z. Teng, Jiemin Wang
Aug 10, 2020
Citations
0
Influential Citations
12
Citations
Journal
ACS sensors
Abstract
Pantetheinase (Vanin-1) is an ectoenzyme, which involves the metabolic pathway of coenzyme A (CoA), can decompose pantetheine into pantothenic acid (CoA precursor) and aminothiol cysteamine. Previous studies have revealed that Vanin-1 with essential biological functions is closely related to many diseases. However, the lack of simple and effective detection methods has severely hindered the further study of Vanin-1's physiological functions. In this work, we have developed a near-infrared (NIR) emission ratio fluorescent probe TMN-PA (I645 nm / I568 nm) that enables us to detect the Vanin-1 rapidly (in 15 min) with a minimum detection limit of 0.37 ng/mL. What's more, this probe shows excellent potential in situ real-time monitoring of the endogenous Vanin-1, contributing to further research on Vanin-1 and understanding its mechanisms in physiological pathology. To our knowledge, this probe is the NIR emission ratio (I645 nm / I568 nm) fluorescent probe never-reported to monitor the activity of Vanin-1 in vivo.