E. Sundby, M. Zotti, T. Anthonsen
Jan 2, 2003
Citations
0
Influential Citations
5
Citations
Journal
Journal of Molecular Catalysis B-enzymatic
Abstract
Enantioselective reductions of ethyl 3-oxobutanoates with fermenting cells or acetone treated cells of Geotrichum candidum gave 3-hydroxyesters with different ee and different predominant configurations depending on reaction conditions. Ethyl 4-bromo-3-oxobutanoate was reduced with APG4 and NADH to give predominantly ethyl (R)-4-bromo-3 hydroxybutanoate while the (S)-configuration was predominant when NADPH was the cofactor. Moreover, when the catalyst was heated before the reaction, the ee was increased indicating that the enzyme giving the (S)-alcohol is more thermolabile than the other.