GawandPratish, MahadevanRadhakrishnan
Apr 15, 2014
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Journal
Industrial Biotechnology
Abstract
Abstract D-ribose is a commercially important functional sugar used as a nutritional supplement and as a starting compound in the synthesis of antiviral drugs. In this study, we report engineered Escherichia coli mutants that can produce D-ribose from glucose and xylose. Two endogenous haloacid dehalogenase-like (HAD) phosphatases from E. coli, HAD12 and HAD13, encoded by the genes ybiV and yidA, respectively, were expressed in E. coli wild type and the glucose-xylose co-utilizing mutant LMSE2. All the mutants constructed in this study produced D-ribose. The mutant RB-006 (LMSE2 expressing ybiV) showed the highest D-ribose titer of 1.16 g/L from 5 g/L each of glucose and xylose. Additionally, using xylose feeding, D-ribose titer was improved to 3.36 g/L. Xylulose and acetate were formed as the major byproducts in the fed-batch study. This study represents the first example of engineered E. coli for production of D-ribose. This study also demonstrates reengineering of a glucose-xylose co-utilizing mutant o...