G. Thorpe, S. Moseley, L. Kricka
1985
Citations
0
Influential Citations
29
Citations
Journal
Analytica Chimica Acta
Abstract
Abstract The benzothiazole derivatives, 2-cyano-6-hydroxybenzothiazole, 6-hydroxybenzothiazole and dehydroluciferin, enhance light emission from the horseradish peroxidase-catalysed oxidation of cyclic diacylhydrazides such as luminol. The relatively intense and prolonged light emission from reactions enhanced by benzothioazole derivatives is easily detected and is utilised in a rapid assay for specific antibody against cytomegalovirus done on black polystyrene microtitre plates. Rapid measurements are possible when a prototype manually-operated microtitre plate reader is used. Light emission from individual wells was quantified by an end-window photomultiplier tube positioned either just above the microtitre plate surface, or some distance away, the light being collected through a fibre optic light guide. The assay was also done on transparent (poly(vinyl chloride) microtitre plates with simultaneous measurement of light emission from several wells; this was achieved with simple instrumentation and a 20 000-ASA Polaroid instant photographic film.