D. Thakore, A. Srivastava, A. Sinha
2012
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Abstract
Ajmalicine is an important antihypertensive obtained from Catharanthus roseus. Transformed hairy roots were generated for the plant which had the ability to accumulate increased concentrations of ajmaciline. These roots can serve as the parent cell line for the economic mass in vitro production of ajmaciline, if cultivated in the appropriate media and right bioreactor configuration. The medium for hairy root cultivation was statistically optimized and thereafter the roots were subjected to different stress conditions primarily to influence the final metabolite accumulation. The objective of the present study was to investigate the effect of five stress factors mannitol, sodium chloride, potassium chloride, cadmium chloride and PVP polyvinyl pyrrolidone K-30 on growth and ajmalicine accumulation under in-vitro mass hairy root cultivation of Catharanthus roseus. Ajmalicine accumulation in actively growing hairy root was increased by addition of PVP to 2.53 mg/gDW(182 % increase than blank control) and by KCl to 4.09 mg/gDW(227 % increase). The maximum secretion of ajmalcine in the medium for mannitol, cadmium chloride, PVP and NaCl was 5.4 mg/l, 1.74 mg/l, 2.192 mg/l and 2.02 mg/l respectively as opposed to 1.32 mg/l in blank control. The mechanisms responsible for these stress effects are discussed herein.