J. V. Staros, Rick W. Wright, Deborah M. Swingle
Jul 1, 1986
Citations
24
Influential Citations
850
Citations
Quality indicators
Journal
Analytical biochemistry
Abstract
Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysulfosuccinimide to such reactions can greatly enhance the yields obtained.