E. Zandvoort, E. Geertsema, W. Quax
Jun 18, 2012
Citations
1
Influential Citations
28
Citations
Journal
ChemBioChem
Abstract
Double play: The enzyme 4-oxalocrotonate tautomerase (4-OT) catalyzes not only the initial cross-coupling of acetaldehyde and benzaldehyde to yield 3-hydroxy-3-phenylpropanal, but also the subsequent dehydration of this aldol compound to yield cinnamaldehyde as the final product. Mechanism-inspired engineering provided an active site mutant (F50A) with strongly enhanced aldol condensation activity.