L. Ke
2008
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Journal
Journal of Molecular Catalysis
Abstract
Enantioseletive hydrolysis of ethyl mandelate was studied by using kinds of enzymes in ionic liquids. N435 exhibited excellent activity and enantioselectivity in the system containing [BMIM]Br compared to several other enzymes and ionic liquids tested. Effects of various parameters such as ionic liquid concentration, substrate concentration, reaction time, reaction temperature, pH and shaking rate were studied. The optimum substrate concentration, ionic liquid concentration, pH, enzyme loading, reaction temperature, shaking rate and reaction time were 0.35 mol/L, 1.71 mol/L [BMIM]Br, pH 7.5, 10 mg N435, 30 ℃, 150 r/min , and 6 h, respectively, under which the enantiomeric excess(ee) and yield of production could reach 58.78% and 65.23%, respectively. The ionic liquid [BMIM]Br was reused by purifying with ethyl acetate.