M. Lindqvist
Mar 13, 2009
Citations
1
Influential Citations
77
Citations
Quality indicators
Journal
Acta pharmacologica et toxicologica
Abstract
Brain tissue is extracted with perchloric acid. The pH of the extract is adjusted to 2.5 and passed through a Dowex 50 W-X4 column. 5-Hydroxy-3-indole acetic acid is eluted with 60 % aqueous methanol, and then 5-hydroxytryp-tophan is eluted with 0.1 M phosphate buffer, pH 6.5. Fluorometric assays are performed according to the method for 5-hydroxytryptamine described by Andeen & Magnusson (1967). The results are satisfactory with regard to reproducibility, recovery, specificity, and sensitivity. If the above procedure is combined with that published by Atack & Magnusson (1970), then 7 different compounds can be estimated after a single column procedure.