Pankaj Dagur, M. Ghosh
Feb 24, 2022
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Quality indicators
Journal
Anti-cancer agents in medicinal chemistry
Abstract
BACKGROUND Laccifer lacca (Kerr) produces a mixture of polyhydroxy anthraquinones (laccaic acid) known as lac dye. Literature suggests that these laccaic acids have its structural resemblance with the anticancer drug Adriamycin (ADR). Hence, it may possess potential anticancer activity. METHOD This study was designed to explore the in vitro anticancer activity of the three fractions of lac dye, i.e., chloroform (C), methanol (M) and water (W) fractions and isolation of constituents from bioactive fraction. RESULTS SRB (Sulforhodamine B) assay method was employed to evaluate the inhibitory action of all the three fractions. However, only methanolic showed promising inhibitory action with GI50 <10, this runs parallel to Adriamycin inhibition (GI50 <10). Two active constituents of the methanolic extract were isolated by using column chromatography and were characterized using UV (UV visible spectrophotometer), IR (Infrared spectroscopy), NMR (Nuclear magnetic resonance) and Mass spectrometry methods. Final structure of the isolated constituents (Laccaic acid D and Laccaic acid B) was confirmed by 13C and 2D NMR data.. CONCLUSION Conclusively, only the methanol fraction (M) showed quiet promising anticancer activity against in vitro MDA-MB-231 and SiHa cell lines compared to the standard adriamycin.