C. Irving, H. Gutmann, ANDD. M. Larson
Dec 1, 1963
Citations
0
Influential Citations
16
Citations
Journal
Cancer research
Abstract
Summary To test the hypothesis that o-hydroxy amines are proximate agents in chemical carcinogenesis paraffin pellets containing the hydrochlorides of 2-amino-1-fluorenol, 2-amino-3-fluorenol, 2-amino-5-fluorenol, 2-amino-7-fluorenol, 2-fluorenamine, and 1-amino-2-naphthol were implanted into the urinary bladders of Swiss female mice. Paraffin pellets alone were implanted as controls. Histological examination of the bladders 40 weeks after implantation showed no evidence of carcinoma. Inflammatory changes, squamous metaplasia, and epithelial hyperplasia were noted with equal frequency in the control and experimental animals. The stability of implanted 2-amino-1-fluorenol-1-C14 hydrochloride and 1-amino-2-naphthol-H3 hydrochloride and the elution of these compounds from the pellets were examined by radioactive tracer methods. Implanted 2-amino-1-fluorenol-1-C14 hydrochloride was stable, whereas 1-amino-2-naphthol-H3 hydrochloride decomposed soon after implantation. The severe irritation of the mouse bladder by paraffin raises the question whether paraffin is a suitable vehicle in the bladder implantation test.