J. Rivier
Dec 19, 1980
Citations
0
Influential Citations
47
Citations
Journal
Journal of Chromatography A
Abstract
Trialkylammonium phosphate or formate and more specifically triethyl- ammonium phosphate or formate buffers (pH < 3 )in the presence of 15–30% acetonitrile have been shown to be compatible with the protein analysis column PAC I-125 of Waters Associates for gel permeation of peptides and proteins. A linear relationship between log molecular weight (1,000–44,000) versus retention time is obtained. The effect of salt and organic modifier concentrations as well as temperature and column load was studied using mix of proteins and peptides of varying iso-electric points as well as hydrophobicity. The advantages of that system (beside being already widely used in reversed-phase high-performance liquid chromatography) include low ionic strength, UV transparence and compatibility (after elimination of the volatile acetonitrile) with most biological systems. Applications include purification of iodinated trace and natural products and precise molecular weight determination.