A. Ravn-Jonsen
Mar 13, 2009
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Quality indicators
Journal
Acta pharmacologica et toxicologica
Abstract
Anaesthetic doses of enibomal (500–1000 mg intravenously) were administered to eight patients and the urine subjected to thin-layer chromatography. Four metabolites were detected, but no enibomal. 5-acetonyl-5-isopropylbarbituric acid (VIII) was demonstrable for up to 6 days, and another metabolite, 1-methyl-5-(2′-oxo-3′-hydroxypropyl)-5-isopropylbarbituric acid (VI), was almost equally long lasting. 1-methyl-5-acetonyl-5-isopropylbarbituric acid (II) and ibomal (NFN) (VII) were present more sporadically, and only during the first 2–3 days. VIII was identified by means of IR-spectrophotometry. The identity of VI was confirmed on three TLC-systems by comparison with synthetic VI. The alteration in the UV-spectrum after exposure to ammonia was similarly used for identification. The identity of II was determined by TLC and UV-spectrophotometry, while VII was determined exclusively by its location on the TLC-system. Quantitative analysis of the urine from four patients, showed VI and VIII to be the main excretion products (6–9 per cent and 2–7 per cent of the dose given). II constituted no more than 0.3–0.9 per cent, while VII occurred in such a small amount that quantitative determination was impossible. The investigation showed that enibomal is deposited in the organism, but not whether its short-term action is due to deposition or to rapid metabolism.