Maulin Patel, Sunu S. Nair A. M. Darji
2013
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Influential Citations
5
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Journal
Journal of Bioremediation and Biodegradation
Abstract
Using the modified base mineral medium containing 100 mg/L of Disperse Orange 3, a decolorizing bacterium with higher decolorization activity was isolated and it showed a decolorization zone of 10 mm; this decolorizing bacterium was identified as Pseudomonas spp. ETL-2013 based on physiological and biochemical characteristics as well as 16s rRNA sequence. In this study, the effects of Pseudomonas spp. ETL-2013's decolorizing ability, for example, glucose concentration, temperature, O 2 , pH and dye concentration, were investigated. The results show that under 40 ° C, pH 7.0, glucose concentration 0.2%, O2 absence and concentration of Disperse Orange 3 of 30 mg/L, the decolorization rate in 24 and 48 h was 86.3 and 93.2%, respectively. The azoreductase produced by Pseudomonas spp. ETL-2013 was extracellular and induced according to electrophoresis experiments and decolorization tests. After purification by ion exchange and gel chromatography, its molecular weight was estimated to be 28,000 Da by SDS-PAGE.