Xu Bin
2011
Citations
0
Influential Citations
0
Citations
Quality indicators
Journal
Journal of Nanjing University of Traditional Chinese Medicine
Abstract
OBJECTIVE To explore the method of determining curcumin pharmacokinetics by more advanced in-vivo imaging of small animals at present to establish experimental schema and evaluate its application.METHODS CY7 fluorescent dyes were applied to label and purify curcumin,and high-performance liquid chromatography(HPLC) was used to determine.After naked mice were injected,fluorescent in-vivo imaging detection was performed to get their real-time images on the distribution of drugs,and fluorescence imaging was made after hearts,livers,spleens,lungs as well as kidneys were dissected,then slices were froze to carry on confocal microscopy observation.RESULTS The label of CY7 was successful,whose purity was more than 90%.Each organ and the bladder can present better concentration metabolic regularity after in-vivo imaging in the body.The condition of drugs into slices can be significantly observed after cutting into slices.CONCLUSION This experimental scheme is feasible,convenient and economical,which has important application value for the pharmacokinetic detection of Chinese drugs.