V. Efimov, A. Fradkov, A. L. Kalinkina
1995
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Journal
Bioorganicheskaia khimiia
Abstract
Plasmid vectors providing a high level expression in Escherichia coli cells of genes for heterologous polypeptides and proteins fused to peptides containing homohistidine clusters have been constructed. The obtained vectors have been used to achieve expression of genes for streptavidin, proinsulin and calcitonin. The hybrid proteins were isolated by affinity chromatography on Ni-agarose columns.