R. KoreAnilkumar, SenthilvelanAnnamalai, SrinivasanBalasubramanian
Mar 19, 2013
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Influential Citations
9
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Journal
Canadian Journal of Chemistry
Abstract
A simple, straightforward, reliable, and efficient protection-free “one-pot, three-step” chemical synthesis of 7-deaza-2′-deoxyguanosine-5′-triphosphate (7-deaza-dGTP) is described. The reaction involves monophosphorylation of 7-deaza-2′-deoxyguanosine using phosphorous oxychloride as the phosphorylating agent, followed by reaction with tributylammonium pyrophosphate, and subsequent hydrolysis affords 7-deaza-2′-deoxyguanosine-5′-triphosphate in a 52% yield with high purity (>99.9%).