J. Inscoe, J. Daly, J. Axelrod
Aug 1, 1965
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Influential Citations
64
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Journal
Biochemical pharmacology
Abstract
Abstract The effect of 3,4-benzpyrene and cold stress on the enzymatic formation of O-methylated dihydroxy compounds from monohydroxy compounds in rats was investigated. Enzyme activity was increased in treated rats only when monohydroxy N-acetyl derivatives (N-acetylserotonin, N-acetyltyramine, and N-acetyl- p -amino-phenol) were used as subtrates. The effect of cold stress and 3,4-benzpyrene was shown to be on the hydroxylating enzyme in the microsomes. Pretreatment with phenobarbital increased, while serotonin decreased, the formation of the O-methylated dihydroxy metabolite of N-acetylserotonin. An enzyme was found in liver microsomes that could O-methylate catechols and that differed from soluble catechol O-methyltransferase in its response to 3,4-benzpyrene and cold stress treatment as well as in pH optimum.