P. Jenner, J. Gorrod, A. Beckett
Sep 1, 1973
Citations
0
Influential Citations
30
Citations
Quality indicators
Journal
Xenobiotica; the fate of foreign compounds in biological systems
Abstract
Abstract1. Cotinine and the diastereoisomeric nicotine-1-N-oxides were the major in vitro metabolites of R-(+)- and S-(-)-nicotine produced by various guineapig hepatic preparations; metabolism was greatest in the 10 000 × g supernatant fraction, being enzymic and NADP(H) dependent. The effect of various substrate concentrations and incubation times on metabolism in the 10 000 × g supernatant fraction was investigated.2. The ratio of the diastereoisomeric N-oxides formed was markedly stereoselective but the formation of cotinine and disappearance of nicotine were not.3. Cotinine formation, but not the disappearance of nicotine or formation of nicotine-1′-N-oxide, was inhibited by SKF 525A and KCN.