B. Fiszer-Szafarz, D. Szafarz, A. Guevara de Murillo
1981
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0
Influential Citations
240
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Quality indicators
Journal
Analytical biochemistry
Abstract
Abstract A fluorometric method using 3,5-diaminobenzoic acid for DNA determination in tissues, cultured cells, nucleated blood cells, and yeast cells is described. The method is general, simple, and rapid, and does not require prior DNA extraction, since tissue is directly solubilized in Triton X-100 and ammonia. The procedure is highly sensitive, and is able to measure rather accurately as little as 10 ng of DNA. It is applicable to all types of DNA structure. The DNA content determined in various tissues and cells was: 2.50 mg/g fresh rat liver, 3.32 mg/g rat diethylnitrosamine-induced hepatoma, 2.49 mg/g fresh mouse liver, 8.76 μg/10 6 human leukocytes, 3.37 μg/10 6 chicken fibroblasts, 2.97 μg/10 8 haploid yeast cells, and 2.84 μg/10 8 haploid yeast protoplasts.