D. Voronkov, A. A. Lyzhin, Yu. V. Dikalova
Jul 1, 2021
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Cell and Tissue Biology
Abstract
L-Aminoadipic acid (L-AA) is known to have toxic effects on astroglia. The purpose of the work is to characterize the morphological changes in astrocytes in vitro and in vivo under the influence of L-AA. The effect of L-AA in the concentration range 0.17–1.4 mM on astrocytes was evaluated in primary dissociated cultures of the rat cerebral cortex and cerebellum, as well as upon stereotaxic injection (20 μg) into the striatum of rats. Concentrations of 0.35–1.4 mM L-AA caused a decrease in the expression of acidic glyofibrillar protein (GFAP), damage and death of astrocytes, pyknosis, and activation of lysosomes (increased LAMP2 expression). On the second day after the injection of L-AA into the striatum of rats, an extensive lesion area devoid of GFAP-positive staining was formed. The data obtained showed that the use of L-aminoadipic acid is promising for modeling damage to astroglia in neurodegenerative diseases.