A. Scholten, U. Brinkman, R. W. Frei
Nov 1, 1981
Citations
0
Influential Citations
24
Citations
Journal
Journal of Chromatography A
Abstract
Abstract The fluorescamine (Fluram) reaction for primary amines has been used for the post-column derivatization of three chloroanilines at relatively low pH values. Separation was carried out on a CN-modified bonded phase with acetonitrile-water (pH 3) as the mobile phase. Various types of reactors (tubular, packed-bed and air-segmented) and mixing tees have been studied and compared for their suitability in post-column reactor systems. Under optimal conditions, detection limits for the derivatized chloroanilines are in the 100-pg range, which is about 10-fold better than with UV detection. The linearity of the calibration graphs is excellent ( r = 0.9999–1.0000) over a concentration range of 2–3 orders of magnitude.