B. Du, Ying Li, Xiaotian Li
Aug 28, 2009
Citations
0
Influential Citations
6
Citations
Journal
Chromatographia
Abstract
Abstract2-Methoxyestradiol is currently in phase II clinical trials as a chemotherapeutic agent. An LC method with fluorescence detection was developed for determination of methoxyestradiol in rat lung. Sample was extracted with ethyl acetate and separated on a C18 column (150 mm × 4.6 mm, 5 μm) with a mobile phase consisting of potassium dihydrogen phosphate-acetonitrile-triethylamine (55:45:0.3, v/v/v, pH 3.0). The excitation and emission wavelength were set at 285 and 325 nm, respectively. Standard curves were linear over the concentration range of 0.25–64 μg g−1. The method was found to be precise, accurate and specific and can be applied to tissue pharmacokinetics of methoxyestradiol in rats.