Aiqin Gong, Xiashi Zhu, Yanyan Hu
Oct 15, 2007
Citations
1
Influential Citations
136
Citations
Quality indicators
Journal
Talanta
Abstract
A new spectrofluorimetric method to determine epristeride (EP) has been developed, which based on the EP has a strong ability to quench the intrinsic fluorescence of bovine serum albumin (BSA). There was the relationship between the fluorescence quenching intensity of BSA (DeltaF=F(BSA)-F(BSA-EP)) and the concentration EP. The quenching mechanism was investigated with the quenching type, the association constants, the number of binding sites and basic thermodynamic parameters. The method had been successfully applied to the analysis of EP in real samples and the obtained results were in good agreement with the results of official method-HPLC.