M. Reiter
Mar 1, 1997
Citations
0
Influential Citations
3
Citations
Journal
Journal of Freshwater Ecology
Abstract
ABSTRACT The fluorescent protein stain 5-(4, 6-dichlorotriazin-2-yl) aminofluorescein (DTAF) has been used to mark bacteria in grazing studies. I modified and simplified the procedure for use with algae. A mixed assemblage of algal genera (Oscillatoria, Ulothrix, Selenastrum, Ankistrodesmus, and Synedra) was exposed to die stain for 20 hours in the dark at room temperature. Stained cells appeared apple-green in color when observed with a UV microscope, and were distinguishable from each other and from a mixture of unstained algae. While there was some difficulty with solubility, it appears that DTAF can be adapted for use with algal cells.