H. Khalfan, R. Abuknesha, D. Robinson
1983
Citations
0
Influential Citations
10
Citations
Journal
The Biochemical journal
Abstract
A method for the preparation of casein labelled with the 4-methylumbelliferyl fluorophore is described, and the product was used as a fluorigenic macromolecular substrate for a sensitive assay of the activity of proteinases. Nanogram quantities of trypsin, chymotrypsin, elastase and cathepsin D can be detected, but the substrate is unaffected by cathepsin B.