H. Fujino, S. Goya
Feb 25, 1990
Citations
0
Influential Citations
6
Citations
Journal
Chemical & Pharmaceutical Bulletin
Abstract
Amantadine was derivatized quantitatively into a fluorescent compound through the amino group by treatment with 3-(4,6-difluorotriazinyl) amino-7-methoxycoumarin (FAMC) in benzene at 140 o C for 15 min. The derivative was subjected to HPLC on TSK gel ODS-120T (250×4.6 mm i.d.) with acetonitrile-water (5:1) as the mobile phase and monitored by fluorescence detection (Ex. 345 nm; Em. 410 nm). The limit of detection was 250 fmol in 100 μl of urine. This method was satisfactory with respect to recovery and precision to quantify amantadine spiked in urine