Y. Tamaura, K. Todokoro, M. Ikebe
Jan 15, 1975
Citations
0
Influential Citations
5
Citations
Journal
FEBS Letters
Abstract
Since Weigele et al. [ 11 synthesized 4-phenylspiro [furan(3H), l’-phthalan] -3,3’-dione, fluorescamine, which reacts specifically with primary amine to yield highly fluorescent product, Udenfriend et al. [2-41 applied this reagent to fluorometric assay of ammo acids, peptides and proteins. In order to clarify the higher order structure of proteins and enzymes, a number of chemical modification reagents which discriminate the states of amino acid residues have been explored by many investigators [5,6]. The reaction of fluorescamine with a substance containing amino groups proceeds almost instantaneously at a room temperature and in aqueous medium. Fluorescamine might be, therefore, the most suitable reagent for discriminating the states of amino groups in the protein molecule. In the present paper it is shown that the states of amino groups in insulin, lysozyme and asparaginase are discriminated by measuring the fluorescence intensity of the product formed by the reaction of fluorescamine with each protein.