H. Kohno, H. Kubo, Koichiro Furukawa
Sep 1, 1991
Citations
0
Influential Citations
13
Citations
Journal
Therapeutic Drug Monitoring
Abstract
A rapid, simple, and accurate method has been developed for the determination of isoniazid and its metabolites (isonicotinic acid, isonicotinyl-glycine, and acetylisoniazid) in human urine by high-performance liquid chromatography. Isoniazid and its metabolites are separated by reversed-phase ion-exchange chromatography with a mobile phase containing hydrogen peroxide as a fluorogenic reagent and butanesulfonate as a hydrophobic ion exchanger, and are detected by fluorometry (excitation at 317 nm and emission at 415 nm) using in-line derivatization at high temperature (160°C). The detection limits are isonicotinic acid, 0.5 μmol/L; isonicotinylglycine, 1 μmol/L; acetylisoniazid, 1 μmol/L; and isoniazid, 1.5 μmol/L. This method can be applied for acetylator phenotyping.