C. Kappler, F. Goltzené, M. Lagueux
1986
Citations
0
Influential Citations
34
Citations
Quality indicators
Journal
International journal of invertebrate reproduction and development
Abstract
Summary Follicle cells have been dissected from vitellogenic ovaries and isolated by collagenase treatment and centrifugation. The cell pellets were incubated with high specific activity tritiated 2-deoxyecdysone and 2,22,25-trideoxyecdysone. Both molecules were readily converted to ecdysone. A privileged sequence of hydroxylations was evidenced for the trideoxyecdysteroid: C-25; C-22; C-2. A significant amount of 2-deoxyecdysone and ecdysone was esterified to Helix hydrolysable conjugates both by the isolated follicle cells and by ooplasm preparations. In the ooplasm, the C-2 hydroxylase, but not the C-22 and C-25 hydroxylation systems, was found to be active. A relatively low level of C-20 hydroxylation was observed in the follicle cells.