J. Zarb, D. Walters
Oct 1, 1994
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0
Influential Citations
17
Citations
Quality indicators
Journal
Letters in Applied Microbiology
Abstract
Radiolabeled products that co‐chromatographed with authentic standards of cadaverine, aminopropylcadaverine (APC) and N,N bis (3‐aminopropyl) cadaverine (3 APC) were isolated following the decarboxylation of a [U‐14C] lysine substrate by fungal lysine decarboxylase extracts. The identity of 3 APC was confirmed by nuclear magnetic resonance (NMR) spectroscopy. The inhibition of the enzymes S‐adenosylmethionine decarboxylase (AdoMetDC) and spermidine synthase led to significant reductions in the recovery of radiolabeled 3 APC. These results show that a range of ectomycorrhizal and plant pathogenic fungi can convert lysine into the higher homologues of cadaverine. These cadaverine homologues appear to be formed via the action of AdoMetDC and spermidine and spermine synthases, although the operation of an additional route for the biosynthesis of these compounds from L‐aspartic‐β‐semialdehyde is a possibility.