C. Hiemke, G. Kauert, DIeter Abbo Kalbhen
Jun 11, 1978
Citations
0
Influential Citations
16
Citations
Journal
Journal of Chromatography A
Abstract
Abstract The gas chromatographic properties of the biogenic amines, catecholamines, phenylethylamines and indolalkylamines as their propionyl derivatives were studied. These derivatives are readily formed in an aqueous medium. Propionylated amines are more stable than their parent compounds and increasingly lipophilic, so that they can be extracted quantitatively into an organic solvent. The propionyl derivatives of the biogenic amines show good gas chromatographic properties. They can be well separated on OV-101 and OV-17 silicones. Care must be taken of certain interactions of the compounds during the chromatographic procedures. Pre-treatment of the column with thionyl chloride inhibits decomposition of β-O-propionylated catecholamines and prevents their interference with other amines. Propionylation is a useful means for the isolation and determination of a wide range of biogenic amines from biological materials by gas chromatography.