A. Dunger, H. Limbach, K. Weisz
Oct 1, 2000
Citations
0
Influential Citations
49
Citations
Journal
Journal of the American Chemical Society
Abstract
NMR studies have been performed on the association of 3',5'-di-O-acetyl-2'-deoxyuridine with a 2'-deoxyadenosine derivative in solution. Using a CDClF2/CDF3 solvent mixture, measurements at very low temperatures allowed the observation of individual complexes in the slow-exchange regime. From 2D NOE connectivities of the imino proton resonances at 125 K the predominant species could be assigned to a Watson- Crick geometry. However, concentration-dependent 15 N chemical shifts of specifically labeled uridine and adenosine nucleosides indicate a considerable population of Hoogsteen base pairs at ambient temperatures. These results together with a more downfield chemical shift of the Watson-Crick bound imino proton point to a stronger hydrogen bond in Watson-Crick when compared to Hoogsteen base pairs.