L. Cegelski, J. Schaefer
Nov 25, 2005
Citations
1
Influential Citations
39
Citations
Journal
Journal of Biological Chemistry
Abstract
Solid-state 13C NMR measurements of intact soybean leaves labeled by 13CO2 (at subambient concentrations) show that excess glycine from the photorespiratory C2 cycle (i.e. glycine not part of the production of glycerate in support of photosynthesis) is either fully decarboxylated or inserted as 13C-labeled glycyl residues in proteins. This 13C incorporation in leaf protein, which is uniformly 15N labeled by 15NH415NO3, occurs as soon as 2 min after the start of 13CO2 labeling. In those leaves with lower levels of available nitrogen (as measured by leaf nitrate and glutamine-glutamate concentrations), the excess glycine is used primarily as glycyl residues in protein.