M. Rohrbach
1978
Citations
1
Influential Citations
96
Citations
Quality indicators
Journal
Analytical biochemistry
Abstract
Abstract [ Glycine -1- 14 C]hippuryl- l -histidyl- l -leucine was synthesized and evaluated as a substrate for the radiochemical assay of angiotensin converting enzyme. Hydrolysis is measured by quantitation of the liberated [ glycine -1- 14 C]hippuric acid by liquid seintillation counting and is linear up to 30% hydrolysis. The advantages of the radiochemical assay over the spectrophotometric quantitation of the liberated hippuric acid are its increased sensitivity and lack of interference by nonionic detergents or lipids.