D. Pramanick, J. Forstová, L. Pivec
Feb 1, 1976
Citations
1
Influential Citations
26
Citations
Journal
FEBS Letters
Abstract
Guanidine hydrochloride (GHC1) was used for isolation of RNA and DNA using repeated precipitation of nucleic acids from 4 M GHC1 by ethanol [ 1 ]. Molecular sieve chromatography in GHCI has been previously used in our laboratory for isolation of high-molecular-weight calf thymus DNA [2] and E. coli R factor DNA [3]. In this paper the general improved procedure is described for isolation of large quantities of DNA from different sources using 4 M GHC1. Different conditions for lysis, nuclease inhibition and deproteinization using two-phase systems of organic solvents and GHCI are demonstrated. The selectivity of precipitation of DNA, RNA and polysacharide using different organic precipitants in 4 M GHCI is also discussed.