M. Yamaguchi, S. Hara, R. Matsunaga
Aug 1, 1985
Citations
0
Influential Citations
24
Citations
Journal
Analytical Sciences
Abstract
Several fluorogenic reagents, 4-bromomethyl-7methoxycoumarin', 9,10-diaminophenanthrene2, 9-anthryldiazomethane3, 9-aminophenanthrene4, 1-bromoacetylpyrene5 and 4-bromomethyl-7-acetoxycoumarin6 have been developed for the determination of carboxylic acids by high performance liquid chromatography (HPLC). In general, these reagents do not permit the determination of the acids at sub-femtomole level. We have developed 3-bromomethyl-6,7-dimethoxy-l-methyl-2(Ihl)-quinoxalinone (Br-DM EQ; Chart 1) as a new fluorogenic reagent for carboxylic acids in HPLC. Linear saturated fatty acids (C3-C20 acids) were employed as model carboxyl compounds for the investigation.