R. Gatti, V. Cavrini, P. Roveri
May 16, 1990
Citations
0
Influential Citations
29
Citations
Journal
Journal of Chromatography A
Abstract
Abstract The use of the methyl ester of 4-(6-methoxynaphthalen-2-yl)-4-oxo-2-butenoic acid as a fluorogenic labelling regent for the high-performance liquid chromatography (HPLC) of biologically important thiols (glutathione, cysteine, acetylcysteine, homocysteine, cysteamine, sodium 2-mercaptoethanesulphonate and thiola) was investigated. The compound reacts selectively and rapidly (10 min at ambient temperature and pH 7.5) with the thiols to give fluorescent adducts that can be separated by reversed-phase HPLC and detected fluororimetrically (λem = 450 nm; λem = 310 nm). Applications to the determination of l -cysteine and mesna in pharmaceutical formulations are described.