F. Vos, G. Slegers
Feb 10, 1995
Citations
0
Influential Citations
2
Citations
Journal
Journal of Chromatography A
Abstract
The high-performance liquid chromatographic determination of [ 11 C]MPGA {(4-[ 11 C]methoxyphenyl)-(5-fluoro-2-hydroxyphenyl)methyleneaminobutyric acid} and [ 11 C]MBENZ (5-fluoro-2-hydroxy-4'-[ 11 C]methoxybenzophenone) is described. The method was successfully applied to the quality control of [ 11 C]MPGA radiopharmaceutical preparations and for plasma analysis after i.v. injection of [ 11 C]MPGA into mice. For determination in plasma, rapid extraction with dichloromethane from buffered plasma (pH 4.5), prior to chromatography, is necessary. Separation is effected on an RP-C 18 column, using a mixture of 0.05 M potassium phosphate-0.025 M citric acid (pH 6.5) and methanol (42:58, v/v) as the mobile phase. Detection is achieved- using an ultraviolet absorption detector set at 255 nm in combination with an NaI (Tl) detector. Quantitative measurements of radioactivity are performed on a one-channel γ-ray spectrometer. The linearity, precision and accuracy for the method are also provided