J. I. Teng, Leland L. Smith
Feb 3, 1995
Citations
1
Influential Citations
20
Citations
Quality indicators
Journal
Journal of Chromatography A
Abstract
Abstract Following oxidation by cholesterol oxidase human erythrocyte oxysterols were analyzed as the corresponding hydroxy- Δ 4 -3-ketones by HPLC with effluent monitoring at 235 nm. Variable amounts of epimeric cholest-5-ene-3s,7-diols, 3s-hydroxycholest-5-en-7-one, cholest-5-ene-3s,19-diol, cholest-5-ene-3s,20-diol, cholest-5-ene-3s,25-diol and cholest-5-ene-3s,26-diol were detected in erythrocyte membranes of patients with sickle cell anemia or sickle cell trait. Only 3s-hydroxycholest-5-en-7-one was detected in erythrocyte membranes of healthy donors.