Aogu Furusho, Mintranee Obromsuk, T. Akita
Oct 20, 2020
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CHROMATOGRAPHY
Abstract
A reversed-phase high-performance liquid chromatographic (HPLC) method using pre-column derivatization with o -phthalaldehyde (OPA) plus N - tert -butyloxycarbonyl- D -cysteine (Boc- D -Cys) has been developed for the determination of aspartic acid (Asp), serine (Ser) and alanine (Ala) enantiomers. D -Amino acids in the real world samples are trace in most cases, and their small peaks should be eluted faster than the huge peaks of the L -forms in order to avoid overlapping. Amino acids were rapidly derivatized at room temperature with OPA plus Boc- D -Cys under simple conditions and were detected by their fluorescence. The target amino acid enantiomers were separated within 60 min on a reversed-phase column, CAPCELL PAK C18 MG II (4.6 x 200 mm), and their resolution values were higher than 2.14. The developed system was successfully validated using standard amino acids, and sufficient calibration lines ( r 2 > 0.9983) and precision (RSD < 5.29%) results were obtained. In a Japanese traditionally fermented amber rice vinegar, all of the target D -amino acids were observed, and their % D values were 21.5 for Asp, 6.8 for Ser and 22.9 for Ala.