K. Payne-Wahl, G. Spencer, R. Plattner
Apr 24, 1981
Citations
0
Influential Citations
38
Citations
Journal
Journal of Chromatography A
Abstract
Abstract Methyl ester, mono-, di- and triglyceride classes were separated and quantitated by high-performance liquid chromatography. Baseline separations were achieved on an amino-, cyano-bonded Partisil column with a gradient from hexane-chloroform (60:65) to hexane-chloroform-acetonitrile (25:65:35). The eluate was monitored by an infrared detector set at 5.72 μm, and peak areas were determined by computer integration. Methyl 9,10-dihydroxystearate was used as an internal standard. The total run time and recycling time was 35 min. A precision of 10% or better was obtained for all components, even at the 1% (w/w) level.