C. Niemann, W. Saenger, R. Nuck
Aug 13, 1991
Citations
0
Influential Citations
15
Citations
Journal
Carbohydrate Research
Abstract
Abstract The distribution of 4-nitrophenyl-α- d -malto-oligosaccharides produced by phosphorolytic synthesis was analyzed by an amine-modified and a C 18 -bonded silica gel. With both systems high resolution of components up to d.p.40 can be achieved by gradient elution using acetonitrile-water and water-methanol, respectively. The use of water-acetonitrile in reversed-phase (C 18 ) chromatography allows only a separation up to d.p. 9. The elution behaviour of oligomers in the d.p. range of 1–10 glucosyl residues per molecule eluted by water-methanol is discussed. The observation of rearrangement products of 4-nitrophenyl-α- d -malto-oligomers was successful by normal-phase chromatography.