Wang Jing
2011
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Journal
Physical Testing and Chemical Analysis
Abstract
HPLC was applied to the determination of 3 alkali orange dyestuffs,namely chrysoidine G(CDG), astrazon orange G(AOG) and astrazon orange R(AOR),which were added contrabandly to foodstuffs as colorants.The homogenized sample(20.00 g) was mixed with 2 g of anhydrous Na_2SO_4 and extracted thrice ultrasonically with absolute alcohol(30 mL+30 mL+30 mL).The extracts were combined and evaporated first at 45℃in vacuum and then by N_2-blowing to dryness.The residue was taken up with the mobile phase(mix 0.02 mol·L~(-1) NH_4OAc solution with methanol in the ratio of 39 + 61 by volume) and made up to 5 mL.After centrifugation,the supernatant was used for HPLC analysis by separation on Agilent C_(18) column.Diode array detector was used for the determination of CDG at 430 nm and of AOG as well as AOR at 480 nm.Linearity ranges for the 3 dyestuffs were found to be same between 0.50 to 20.0 mg·L~(-1),with detection limits(3S/N) of 0.10 mg·L~(-1).Tests for recovery and precision were performed by addition of standards to a sample of yellow croaker,and the results of average recovery found were 91.2%,93.6%and 96.0%for the 3 dyestuffs respectively, with RSD's(n=6) less than 2%.