M. J. R. Reddy, V. V. Reddy, B. A. Rao
May 1, 2006
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Journal
Indian Journal of Chemical Technology
Abstract
A simple high performance liquid chromatographic (HPLC) method for the determination of 2-diazonaphthoquinone-5-sulphonic acid phenyl ester (DNQ) is developed. 5-Amino naphthalene-1-sulphonic acid sodium salt is used as internal standard. A Polaris C18-5 μ silica column (250x4.6 mm), 5 μ particle size, was equilibrated with a mobile phase composed of methanol and water (80:20, v/v). Flow rate was 1.0 mL/min. The elution time for 5-amino naphthalene-1-sulphonic acid sodium salt and DNQ was approximately 2 and 4 min, respectively. Calibration curve of DNQ was linear in the concentration range of 10-50 μg/mL. Limit of detection and quantification were measured in the lowest concentrations of the replicates. Recovery is about 96%.