Cheng Hai-bin
2012
Citations
0
Influential Citations
0
Citations
Journal
Chinese Journal of Pharmaceutical Analysis
Abstract
Objective: To establish an HPLC method for rapid screening and simultaneous determination of eight preservatives(benzyl alcohol,phenol,benzoic acid,sorbic acid,benzoic acid,4-hydroxy-,methyl ester,benzoic acid,4-hydroxy-,ethyl ester,benzoic acid,4-hydroxy-,propyl ester,benzoic acid,4-hydroxy-,butyl ester)and benzaldehyde from degradation product of benzyl alcohol in oxymatrine injection.Methods: The analysis was performed on Welch Materials XB-C18 column(4.6 mm×250 mm,5 μm)with the mobile phase consisting of acetonitrile-0.02 mol·L-1ammonium acetate(glacial acetic acid adjustable pH 5.0)in gradient mode.The detection wavelength was set at 211 nm for benzyl alcohol and phenol,225 nm for benzoic acid,248 nm for benzaldehyde and 256 nm for sorbic acid,benzoic acid,4-hydroxy-,methyl ester,benzoic acid,4-hydroxy-,ethyl ester,benzoic acid,4-hydroxy-,propyl ester,1benzoic acid,4-hydroxy-,butyl ester.Result: Nine constituents showed good linearity(r0.9998)in the range of the test concentration.The average recoveries of the method were between 95.6% and 102.2%.Conclusion: The method is sensitive,rapid and accurate with good repeatability,which can be used for rapid screening and determination of the content of preservatives in injection.