C. Hoes, O. V. van Batenburg, K. Kerling
Aug 8, 1977
Citations
0
Influential Citations
7
Citations
Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Summary Two semisynthetic RNase S′ analogues, in which the active site residue histidine-12 is replaced by homohistidine, while in one of them methionine-13 is substituted by isoleucine as well, were studied with respect to their capacity to hydrolyze 2′,3′-cyclic CMP, a model substrate for the second step of the RNase catalyzed breakdown of RNA. Kinetic parameters have been determined at pH 6. From the results obtained it is concluded that replacement of histidine-12 by homohistidine has no drastic effect on the catalytic properties of the enzyme in the second step of the reaction.