T. Bavaro, Pamela Torres‐Salas, D. Ubiali
Apr 30, 2013
Citations
0
Influential Citations
10
Citations
Journal
RSC Advances
Abstract
A purified lipase fraction from Rhizomucor miehei (RML) was immobilized and used to selectively hydrolyze hexa-O-acetyl-lactal 1 to penta-O-acetyl-3-hydroxylactal 2. The reaction was performed in low water content tert-butanol (7% USP water), thus allowing the control of by-product formation. This solvent was superior to the established buffer/acetonitrile system in terms of reagent/product solubility, biocatalyst stability and environmental impact. The reaction was scaled up to 28 g L−1 (50 mM) affording the product 2 in very high yield (85%) and purity (98%).